Viral structure may allow for African swine fever treatmentViral structure may allow for African swine fever treatment
Unique structure of African swine fever virus enzyme may allow for drug development.
March 1, 2017
A DNA-copying protein from a lethal pig virus has a unique structure that may offer a target for drugs designed to combat this important agricultural disease, according to a study published Feb. 28 in the open-access journal PLOS Biology by Yiqing Chen and colleagues at Fudan University in Shanghai, China.
African swine fever virus (ASFV) is a highly contagious and deadly disease in pigs that has spread from Africa to areas of Europe and Asia. Currently, there are no treatments, and control relies on killing entire herds once infection is detected. Viral replication depends in part on a polymerase enzyme, AsfvPolX, that repairs breaks in the DNA, but the structure of this enzyme has not been determined in detail. Here, the researchers used X-ray diffraction and nuclear magnetic resonance to solve the structure at atomic resolution.
The team found that the enzyme contained a unique binding pocket for the building blocks of DNA (nucleotides) that was not seen in related enzymes in other organisms. They also found several other unique structural features, including a pair of hydrophobic amino acids that interact with incoming nucleotides and a "platform" created by two basic amino acids that stabilizes a mismatched nucleotide pair, increasing the rate of incorporation of erroneous nucleotides into the DNA chain during the repair process.
Together, these features give the polymerase its unique character of a high rate of DNA replication combined with a high copying error rate.
Blocking the binding pocket with a drug may be a valuable strategy to treat ASFV infection, the researchers suggested. "Exploiting this unique structural feature to attack the virus may offer a rapid route to develop treatments for this important agricultural virus," said Chen, although he noted one caveat: the high error rate of the AsfvPolX polymerase enzyme means that the virus mutates rapidly and, therefore, may evolve resistance to drugs designed to block it.
The article in PLOS Biology is available at http://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.1002599.
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