Haplotype map of wheat producedHaplotype map of wheat produced
Study of genomic diversity of wheat included 62 lines from around world.
March 27, 2015
KANSAS State University scientists have released findings of a complex, two-year study of the genomic diversity of wheat that creates an important foundation for future improvements in wheat around the world.
Their work has produced the first haplotype map of wheat that provides a detailed description of genetic differences in a worldwide sample of wheat lines. In genetics, a haplotype map is a powerful tool for transferring sequence-level variation to multiple gene mapping projects.
"All of these new, genomic-based strategies of breeding promise to significantly accelerate breeding cycles and shorten release times of future wheat varieties," said Eduard Akhunov, Kansas State associate professor of plant pathology and the project's leader.
Plant scientists often look at the genetic makeup of an organism to breed new varieties for specific, desirable traits, such as drought, pest or disease resistance.
Akhunov said the haplotype map gives scientists quick access to rich, genetic variation data that increase the precision of mapping genes in the wheat genome and improve scientists' ability to select the best lines in breeding trials.
Akhunov's research associates, Katherine Jordan and Shichen Wang, are lead authors of the study, "A Haplotype Map of Allohexaploid Wheat Reveals Distinct Patterns of Selection on Homoeologous Genomes," which will be published in an upcoming issue of the journal Genome Biology.
The project was coordinated through the International Wheat Genome Sequencing Consortium and included groups in the U.S., Canada, the U.K. and Australia. Much of the work took place in Kansas State University's Integrated Genomics Facility.
The study included 62 wheat lines from around the world that were either modern cultivars or varieties not previously improved through formal breeding techniques, called landraces.
To reduce the complexity of the wheat genome, the research team developed a tool called "exome capture assay" to perform targeted sequencing of only the functional parts of the larger wheat genome. This technique bypasses those parts of the genome that are repetitive, according to Akhunov.
The scientists found 1.6 million locations — called single nucleotide polymorphisms — in the genome where the wheat lines differed from one other. The research team used this information to describe the impact of these differences on the function of tens of thousands of wheat genes.
"Once genes controlling agronomic traits are identified, they can be used for improving wheat using not only traditional breeding approaches but also new strategies that are based on biotechnology and molecular biology," Akhunov explained.
"In the future, we will expand the set of wheat lines characterized using our sequencing strategy by including not only more genetically and geographically diverse wheat lines but also by including close and distant relatives of wheat," he said. "These wheat relatives are known for being a reservoir of valuable genes for agriculture that can improve abiotic and biotic stress tolerance or other quality traits and increase yield."
Akhunov said genomics-based approaches are now being introduced into every wheat breeding program worldwide.
"I am sure that we will see the impact of diversity resources developed at Kansas State University on wheat breeding within three to five years," he said.
Funding for the study was provided by the U.S. Department of Agriculture's National Institute of Food & Agriculture through the National Research Initiative.
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