August 7, 2017
The combination of a bacterium that normally lives in the gut and a protein-rich diet promotes a more tolerant, less inflammatory gut immune system, according to new research at Washington University School of Medicine in St. Louis, Mo.
The findings, in mice, suggest a way to tilt the gut immune system away from inflammation.
Immune cells patrol the gut to ensure that harmful microbes hidden in food don’t sneak into the body. Cells that are capable of triggering inflammation are balanced by cells that promote tolerance, protecting the body without damaging sensitive tissues. When the balance tilts too far toward inflammation, inflammatory bowel disease can result.
Now, researchers at Washington University School of Medicine in St. Louis have found that a kind of tolerance-promoting immune cell appears in mice that carry a specific bacterium in their guts. Further, the bacterium needs the amino acid tryptophan to trigger the cells’ appearance.
“We established a link between one bacterial species — Lactobacillus reuteri — that is a normal part of the gut microbiome, and the development of a population of cells that promote tolerance,” said Dr. Marco Colonna, the Robert Rock Belliveau MD professor of pathology and the study’s senior author. “The more tryptophan the mice had in their diet, the more of these immune cells they had.”
If such findings hold true for people, it would suggest that the combination of L. reuteri and a tryptophan-rich diet may foster a more tolerant, less inflammatory gut environment, which could mean relief for the million or more Americans living with the abdominal pain and diarrhea of inflammatory bowel disease.
The study is published Aug. 3 in the journal Science.
Postdoctoral researcher Luisa Cervantes-Barragan was studying a kind of immune cell that promotes tolerance when she discovered that one group of study mice had such cells, while a second group of study mice that were the same strain of mice but were housed far apart from the first group did not have such cells.
The mice were genetically identical but had been born and raised separately, indicating that an environmental factor influenced whether the immune cells developed.
She suspected the difference had to do with the mice’s gut microbiomes — the community of bacteria, viruses and fungi that normally live within the gastrointestinal tract.
Cervantes-Barragan collaborated with Chyi-Song Hsieh, the Alan A. & Edith L. Wolff distinguished professor of medicine, to sequence DNA from the intestines of the two groups of mice. They found six bacterial species present in the mice with the immune cells but absent from the mice without them.
With the help of Dr. Jeffrey I. Gordon, the Dr. Robert J. Glaser distinguished university professor, the researchers turned to mice that had lived under sterile conditions since birth to identify which of the six species was involved in inducing the immune cells. Such mice lack a gut microbiome and do not develop this kind of immune cell.
When L. reuteri was introduced to the germ-free mice, the immune cells arose.
To understand how the bacteria affected the immune system, the researchers grew L. reuteri in liquid and then transferred small amounts of the liquid — without bacteria — to immature immune cells isolated from mice. The immune cells developed into the tolerance-promoting cells. When the active component was purified from the liquid, it turned out to be a byproduct of tryptophan metabolism known as indole-3-lactic acid.
Tryptophan is a normal part of the diet. When the researchers doubled the amount of tryptophan in the mice’s feed, the number of such cells rose by about 50%. When tryptophan levels were halved, the number of cells dropped by half.
“The development of these cells is probably something we want to encourage since these cells control inflammation on the inner surface of the intestines,” Cervantes-Barragan said. “Potentially, high levels of tryptophan in the presence of L. reuteri may induce expansion of this population.”
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