TUM trout virus.jpg Credit: Chair of Zoology / Technical University of Munich
Dr. Jens-Eike Taeubert examining a trout infected by a previously unknown virus, which is related to a virus that infects salmon in the North Atlantic and the Pacific.

Researchers pinpoint cause of brown trout dying

Death losses of trout in Alpine rivers triggered by previously unknown virus related to one that infects salmon in the North Atlantic and Pacific.

Every summer in central Europe, tons of brown trout perish, but now, an interdisciplinary team from the Technical University of Munich (TUM) in Germany has discovered the culprit of the mysterious die-offs affecting brown trout.

The losses are triggered by a previously unknown virus related to a virus that infects salmon in the North Atlantic and the Pacific, according to an announcement from TUM.

The setting is always in the same river sections, and the victims are always brown trout, TUM said. Within a matter of days, their skin turns dark and the animals perish. Researchers and authorities have been hunting down the cause of this mysterious mass mortality for decades — unsuccessfully, until now.

Using cutting-edge analysis methods, professor Ralph Kühn and a research team from TUM have identified the pathogen that triggers the proliferative darkening syndrome (PDS) in brown trout.

"The biggest challenge was identifying a pathogen nobody knew. At the outset, it was not even clear whether it was a bacterium, a virus, a parasite or an environmental toxin," Kühn said.

Ten years of scientific detective work followed. The researchers set up two experimental stations at the Iller river - one in the headwaters, near Obersdorf, Germany, where the fish disease had never been observed; a second one in the lower course near Kempten, Germany, where brown trout die every summer.

At both stations, the researchers set up aquariums fed with river water. From May to September they observed the fish in the aquariums and took tissue samples that were frozen and sent to the TUM laboratory for analysis.

The observations indicated that PDS advances in three phases, TUM said, noting that initially, the fish look healthy. Then changes in internal organs such as the liver and kidneys appear. In the third phase, the brown trout skin turns dark and the animals die shortly thereafter.

"The progression of the disease quickly led us to suspect that PDS was a viral disease," Kühn said.

To detect the virus in the samples, the researchers deployed a set of modern molecular genetic procedures, known as next-generation technology. Genomes can be analyzed rigorously using these procedures.

First, the progression of the disease in the fish was characterized at the gene response level. Subsequently, the researchers looked at animals with a similar immune response to determine the nucleotide sequence of all inherited genetic material — of the fish and its possible pathogens.

Viral discovery

The researchers deployed computer programs to pinpoint the pathogenic virus in this mountain of genetic data. Using deep bioinformatic processing, they successfully identified parts of the pathogen's genetic profile and compared them to the profiles of known viruses, TUM said.

Extended, virus-specific sequencing led to the determination of the viral genome. The result: The culprit behind the demise of the brown trout is a piscine reovirus. It is related to the virus that infects salmon in the North Atlantic and the Pacific, where it causes significant economic damage, TUM said.

In the next step, the researchers hope to grow the virus in the laboratory and investigate why it only occurs in certain sections of the Alpine rivers and to what extent global fish trade facilitates its spread.

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