An Aedes aegypti mosquito prepares to bite a human. U.S. Department of Agriculture.
An Aedes aegypti mosquito prepares to bite a human.

Researchers aim to disrupt egg production in Zika-spreading mosquito

Study focuses on the Aedes aegypti “fat body” that plays a key role in reproduction.

The mosquito Aedes aegypti, which can spread dengue fever, chikungunya, Zika fever and yellow fever virus, requires a blood meal to develop eggs. One way to control the spread of these diseases is to tamper with the reproductive events that follow this mosquito’s blood meal.

This is what a team of scientists at the University of California-Riverside explored at the molecular level. The researchers focused on small regulatory RNA molecules, called microRNAs, that play a critical role in mosquito egg maturation.

They studied microRNA expression in the Aedes aegypti fat body — the metabolic center that plays a key role in reproduction. Since proper functioning of the fat body is essential for the development of the female reproductive system after a blood meal, identifying which microRNAs are important to fat body functions and what specific genes they target can help design ways to manipulate the levels of microRNA or their targets, affect their interactions, disrupt mosquito reproduction and, thus, prevent the spread of diseases the mosquitoes transmit.

In the online Proceedings of the National Academy of Sciences, the researchers reported that they observed five major microRNA expression peaks within a 48-hour period following the female mosquito’s blood meal.

“What we observed is that the levels of many microRNAs change significantly throughout the 48-hour period following a blood meal, indicating that these microRNAs, in turn, may be establishing significant changes in expression of key genes during this time in the fat body,” said Fedor V. Karginov, an assistant professor of cell biology and neuroscience, who co-led the research team along with Alexander S. Raikhel, a distinguished professor of entomology at the University of California-Riverside. “Our work has given us a much needed picture of which microRNAs are abundant in the fat body tissue, how each microRNA subgroup changes over time, and we have confirmation that specific up- and down-regulation of microRNA levels takes place during egg development.”

Specifically, the researchers measured the levels of all microRNAs in the fat body (around 100 different microRNAs) at five points of time, starting just before mosquitos take a blood meal, and then 6, 24, 36 and 48 hours after the blood meal. The timing of these was chosen based on previously known information on the timing of major physiological changes — or milestones — in the fat body after a blood meal.

Karginov explained that each microRNA, together with a partner protein called Argonaute or “Ago,” binds to (or “targets”) several to many messenger RNAs (mRNAs), and thus down-regulates the expression of the corresponding genes. Determining the targets of important microRNAs is crucial to uncover the regulatory gene networks that drive the physiological changes in the fat body after blood meal.

Karginov, Raikhel and their team members experimentally identified the binding sites for Ago/microRNAs on mRNAs in the fat body. They performed this identification at two points of time to study any changes that may have occurred, using “CLIP-seq,” an experimentally challenging procedure that, to Karginov and his team’s knowledge, has not been used on mosquito tissues before, and that provides a large trove of potential microRNA-mRNA interactions for further investigation.

“The CLIP-seq data have given us insight into which genes the microRNA target, providing a solid foundation for future studies of miRNA regulation during the egg production cycle,” Raikhel said. “Now that we know these genes, we are a step closer to controlling the spread of Aedes aegypti by disrupting a key process in the reproductive cycle: egg production.”

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