The USPOULTRY Foundation announced the completion of a funded research project at North Carolina State University in Raleigh, N.C., that has clarified how blackhead disease spreads.

In the project, Dr. Robert Beckstead with the Prestage department of poultry science at North Carolina State University developed diagnostic assays needed for advancing the understanding of blackhead disease. His research shows clearly how the disease most likely spreads and clarifies which cleaning and disinfecting methods are effective in decontaminating facilities and equipment.

Beckstead explained that Heterakis gallinarum (heterakis) is a heavily prevalent but rarely diagnosed avian parasite that poses a silent threat to poultry producers. The eggs of this nematode often act as carriers of a protozoan parasite called Histomonas meleagridis (histomonas) that causes a potentially deadly disease in poultry commonly referred to as blackhead disease.

The prevalence of blackhead disease has increased significantly over the past three decades due to the revocation of Food & Drug Administration approval for use of drugs in commercial poultry that were previously administered to prevent transmission of the protozoan and treat afflicted birds, Beckstead explained.

Poultry producers must adapt their farm management practices to mitigate the chances of transmitting the protozoan between flocks, he added, noting that this may be accomplished by controlling the spread of heterakis. However, preventing flock-to-flock transmission of heterakis on commercial poultry facilities is made difficult by a limited understanding of reservoirs for infection and a lack of information regarding effective decontamination procedures, Beckstead reported.

Beckstead said the objectives of his study were to: (1) develop molecular diagnostic tests for heterakis; (2) identify heterakis disease reservoirs, and (3) develop an assay to test the efficacy of different compounds to destroy heterakis.

A highly sensitive and heterakis-specific polymerase chain reaction (PCR) test was developed and validated, he reported, and this diagnostic PCR was used to demonstrate that both earthworms and darkling beetles may act as potential carriers or vectors of the nematode.

According to Beckstead, heterakis were detected in earthworms collected from backyard chicken flocks and turkey grow-out facilities that were experiencing outbreaks of blackhead disease. These data suggest that testing a known carrier of this nematode can indicate whether heterakis is present at a facility, Beckstead said.

The detection of heterakis DNA from darkling beetle isolates indicates that beetles may be responsible for the transmission of blackhead disease between subsequent flocks, the researcher said, adding that 100% of mature broiler breeders and floor-raised layers sampled during this study were positive for sexually mature heterakis in their ceca. Based on PCR analysis, Beckstead said all populations of heterakis collected were positive for histomonas. Each of these birds are therefore responsible for shedding heterakis eggs into the environment resulting in recontamination of the chicken facility and serve as a source of contamination for other poultry facilities, especially when litter is removed from the facility and spread at other locations.

In another piece of the study, Beckstead developed an assay to test whether chemical treatment or disinfectants are capable of damaging heterakis eggs and rendering them nonviable. Six chemical compounds (i.e., sodium hypochlorite in Clorox Bleach; sodium dichloroisocyanurate; a combination of hydrogen peroxide, N-Alkyl-N,N-dimethyl-N-benzylammonium chloride and diacetin; a combination of propionic acid, acetic acid, ammonium hydroxide, sodium hydroxide and iodine; sodium bisulfate, and a combination of potassium peroxymonosulfate and sodium chloride) in addition to salt, limestone and diesel fuel were tested.

Both sodium hypochlorite and sodium dichloroisocyanurate damaged the heterakis eggshell, but only 15,125 parts per million of sodium hypochlorite was capable of rendering the egg nonviable.

These results demonstrate that the majority of current cleaning protocols are not effective in killing heterakis eggs in poultry houses and indicate the urgent need for product testing to identify farm management protocols to decontaminate farm equipment or poultry houses, Beckstead concluded.