The "jumping genes" of corn, or maize, have been mapped by an international team led by researchers at the University of California-Davis (UC-Davis) and the Cold Spring Harbor Laboratory. The discovery could ultimately benefit the breeding and production of corn, one of the world's most important crops.
Transposable elements, or transposons, are DNA sequences that can move locations within a genome ("jumping genes"). Discovered in corn by Nobel-winning geneticist Barbara McClintock in the 1940s, they were long considered by many scientists to have little role in genetics. However, others, including McClintock, thought that transposons within a genome may play important roles in cells, including regulating gene expression.
Transposable elements are now found in most organisms, making up more than 80% of the maize genome and nearly 50% of the human genome.
Until now, the exact locations of transposons have been elusive, primarily because they have been so difficult to sequence and assemble. UC-Davis graduate student in population biology Michelle Stitzer and maize geneticist Jeff Ross-Ibarra, a professor of plant sciences, worked with colleagues at Cold Spring Harbor and several universities and genome technology companies to create a new corn reference genome that includes the many complex repeat regions.
The new sequencing technology they used was described in a recent Nature publication.
Identifying, classifying transposons
"Earlier maize reference genomes did not identify all of the repetitive regions," Stitzer said. "Until now, we knew relative positions of sequence segments, but not all of the messy parts in between. This new technology has allowed us to sequence all of the repetitive regions."
Stitzer has developed methods to identify the positions of transposons in maize even when they jump into each other.
"The Nature publication focused on the technology, which gave a valuable, high-quality genome sequence," Ross-Ibarra said. "Michelle then created computational algorithms to identify individual transposable elements across the whole genome, which had never been done before.
"Her work is revealing an entire ecology of transposons, complete with complex relationships of competition and cooperation. This is enabling us to begin to understand the rich biodiversity of the genome as an ecosystem," Ross-Ibarra added.
Nathan Springer, a professor at the University of Minnesota and co-author on the Nature paper, noted that Stitzer's "new approaches to identifying and classifying the full complement of transposable elements in maize should lead to new fundamental biological discoveries."
New windows in transposon research
Transposons can regulate and change the expression of nearby genes, depending on where they land in the genome, Stitzer said, adding, "That's very important to know but was difficult to identify when we couldn't figure out where they were in the genome sequence."
Transposon insertions and their impact on gene expression are known to affect the way the corn plant interacts with its environment. For example, different transposon insertions confer drought tolerance, altered flowering time, the ability to grow in toxic aluminum-rich soils and have allowed corn to spread to temperate latitudes by breaking sensitivity to the long days of the tropics.
Broadly, transposable element insertions have been shown to alter gene expression in stressful conditions, but these insertions with known functional consequences only represent a handful of the hundreds of thousands of transposable elements in the corn genome.
Damon Lisch, a professor at Purdue University who studies the regulation and evolution of plant transposable elements, said, "We simply cannot understand the complexity of plant genomes unless we can identify transposable elements. Michelle's work provides an invaluable road map that allows us to begin to untangle the diversity of all of the genetic elements that make up the maize genome."
Ross-Ibarra said now that the corn genome is fully sequenced and transposon locations have been determined, a new realm of research is opening beyond the role of individual genes in maize: determining the role of individual transposons.